BMI is Associated with Serum Leptin and Lipid Profile

Aim: To find a possible association of serum leptin and lipid profile with obesity Place of study: The subjects were selected from Outpatient Department of Internal Medicine, Mayo Hospital and British Sliming Center. Methods: The consent from the patients was taken before their participation in this study. The subjects were classified into two categories; obese (BMI>30) and Non-Obese (BMI<25). The body weight of each individual dressed in light clothing was measured using a carefully calibrated weighing balance. The height of each individual was measured using a vertical-measuring rod; a waist and hip circumference was also measured to calculate waist/hip ratio. Blood samples were taken early in the morning, 12 hours postprandial. About 10 ml of venous blood was drawn from the subjects. The serum samples were stored at 2-5°C for not more than 24 hours prior to lipid profile and plasma Leptin Measurements. Human leptin ELISA kit (ENZO-ALX-850-044-Kl01) was used (that is designed with sandwich enzyme immunoassay method for the quantitative measurement of human leptin). Triacylglycerol concentration was measured by using GPO-PAP method, Triglyceride LABKIT was used for this purpose. Cholesterol concentration was measured by using CHOD/PAP method, Cholesterol LABKIT was used for this purpose. Results: The mean age of the patients was 45.35 ± 5.96 years with min and max age 32-58 years respectively. Total 91 subjects were selected. 43 (47.3%) of them were males and remaining 48 (52.7 %) were females. The results shows positive significant correlation in BMI and Serum Leptin, r=0.316 (0.002), significant correlation in BMI and total Cholesterol, r=0.0.195 (0.000), positive significant correlation in BMI and HDL Cholesterol, r=0.0.108 (0.000), no significant correlation in BMI and LDL cholesterol, r=-0.001 (0.747), no significant correlation in BMI and LDL cholesterol to HDL Cholesterol ratio, r= -.041 (0.270). Conclusion: Based on the results of this study it can be concluded that there exists some association between lipid profile and serum leptin with Body Mass Index. Further studies must be carried out to further investigate how leptin interacts with lipid profile and vice versa